PPM1A antibody

Catalog No. ABIN7466754

This Rabbit Polyclonal antibody specifically detects PPM1A in WB, IF, ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for PPM1A antibody (ABIN7466754)

Target: PPM1A (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPM1A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PPM1A Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PPM1A. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , 293T , A431 , PC-12 , Rat2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PPM1A

Target: PPM1A (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A))

Alternative Name: protein phosphatase, Mg2+/Mn2+ dependent 1A

Background: Protein phosphatase, Mg2+/Mn2+ dependent 1A , PP2C-ALPHA , PP2CA , PP2Calpha,The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

Molecular Weight: 42 kDa

Gene ID: 5494

UniProt: P35813