MRPL12 antibody

Catalog No. ABIN7466760

This Rabbit Polyclonal antibody specifically detects MRPL12 in WB, IF, IHC (p) and ICC. It exhibits reactivity toward Human.

Quick Overview for MRPL12 antibody (ABIN7466760)

Target: MRPL12 (Mitochondrial Ribosomal Protein L12 (MRPL12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL12 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-MRPL12 Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human MRPL12. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , A431

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.56 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MRPL12

Target: MRPL12 (Mitochondrial Ribosomal Protein L12 (MRPL12))

Alternative Name: mitochondrial ribosomal protein L12

Background: Mitochondrial ribosomal protein L12 , 5c5-2 , L12mt , MRP-L31/34 , MRPL7 , MRPL7/L12 , RPML12,Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex. [provided by RefSeq]

Molecular Weight: 21 kDa

Gene ID: 6182

UniProt: P52815