Gephyrin antibody

Catalog No. ABIN7466988

This Rabbit Polyclonal antibody specifically detects Gephyrin in WB, IF, IP, IHC (p), ICC and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Gephyrin antibody (ABIN7466988)

Target: Gephyrin (GPHN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Gephyrin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Gephyrin Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Gephyrin. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3 , JC

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.32 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Gephyrin

Target: Gephyrin (GPHN)

Alternative Name: gephyrin

Background: Gephyrin , GEPH , GPH , GPHRYN , HKPX1 , MOCODC,This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described, however, the full-length nature of all transcript variants is not currently known. [provided by RefSeq]

Molecular Weight: 80 kDa

Gene ID: 10243

UniProt: Q9NQX3

Pathways: Synaptic Membrane, Skeletal Muscle Fiber Development