Iba1 antibody

Catalog No. ABIN7467197

The Mouse Monoclonal anti-Iba1 antibody (Clone GT10312) (ABIN7467197) specifically detects Iba1 in WB, ICC, IF, FACS, IHC (p) and IHC (ff). The antibody is reactive with Human samples.

Quick Overview for Iba1 antibody (ABIN7467197)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Iba1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Free Floating) (IHC (ff))

Clone: GT10312

Product Details anti-Iba1 Antibody

Cross-Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Purification: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Iba1. The exact sequence is proprietary.

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name: allograft inflammatory factor 1

Background: Allograft inflammatory factor 1 , AIF-1 , IBA1 , IRT-1 , IRT1,This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Molecular Weight: 17 kDa

Gene ID: 199

UniProt: P55008

Pathways: Smooth Muscle Cell Migration