Nestin antibody

Catalog No. ABIN7467398

This anti-Nestin antibody is a Rabbit Polyclonal antibody detecting Nestin in WB, ICC and IF. Suitable for Human.

Quick Overview for Nestin antibody (ABIN7467398)

Target: Nestin (NES)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-Nestin Antibody

Cross-Reactivity: Human, Rabbit

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Nestin. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: SH-SY-5Y , SK-N-SH

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.38 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: nestin

Background: Nestin , Nbla00170,Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]

Molecular Weight: 177 kDa

Gene ID: 10763

UniProt: P48681