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Nestin antibody

This anti-Nestin antibody is a Rabbit Polyclonal antibody detecting Nestin in WB, ICC and IF. Suitable for Human.
Catalog No. ABIN7467398

Quick Overview for Nestin antibody (ABIN7467398)

Target

See all Nestin (NES) Antibodies
Nestin (NES)

Reactivity

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  • 32
  • 4
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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This Nestin antibody is un-conjugated

Application

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Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
  • Cross-Reactivity

    Human, Rabbit

    Purification

    Purified by antigen-affinity chromatography.

    Immunogen

    Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Nestin. The exact sequence is proprietary.

    Isotype

    IgG
  • Application Notes

    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    Comment

    Positive Control: SH-SY-5Y , SK-N-SH

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.38 mg/mL

    Buffer

    0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

    Preservative

    Thimerosal (Merthiolate)

    Precaution of Use

    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Target

    Nestin (NES)

    Alternative Name

    nestin

    Background

    Nestin , Nbla00170,Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]

    Molecular Weight

    177 kDa

    Gene ID

    10763

    UniProt

    P48681
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