Nestin antibody
Quick Overview for Nestin antibody (ABIN7467398)
Target
See all Nestin (NES) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Cross-Reactivity
- Human, Rabbit
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Purification
- Purified by antigen-affinity chromatography.
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Immunogen
- Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Nestin. The exact sequence is proprietary.
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Isotype
- IgG
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Application Notes
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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Comment
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Positive Control: SH-SY-5Y , SK-N-SH
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.38 mg/mL
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Buffer
- 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal
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Preservative
- Thimerosal (Merthiolate)
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Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Nestin (NES)
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Alternative Name
- nestin
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Background
- Nestin , Nbla00170,Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]
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Molecular Weight
- 177 kDa
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Gene ID
- 10763
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UniProt
- P48681
Target
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