TFB2M antibody (C-Term)

Catalog No. ABIN7467427

The Rabbit Polyclonal anti-TFB2M antibody has been validated for WB. It is suitable to detect TFB2M in samples from Human.

Quick Overview for TFB2M antibody (C-Term) (ABIN7467427)

Target: TFB2M (Transcription Factor B2, Mitochondrial (TFB2M))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TFB2M antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TFB2M Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TFB2M. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: TFB2M transfected 293T cell

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for TFB2M

Target: TFB2M (Transcription Factor B2, Mitochondrial (TFB2M))

Alternative Name: transcription factor B2, mitochondrial

Background: Transcription factor B2, mitochondrial , Hkp1 , mtTFB2,S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity.

Molecular Weight: 45 kDa

Gene ID: 64216

UniProt: Q9H5Q4