MRPL42 antibody

Catalog No. ABIN7467595

The Rabbit Polyclonal anti-MRPL42 antibody has been validated for WB, ICC and IF. It is suitable to detect MRPL42 in samples from Human.

Quick Overview for MRPL42 antibody (ABIN7467595)

Target: MRPL42 (Mitochondrial Ribosomal Protein L42 (MRPL42))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL42 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-MRPL42 Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human MRPL42. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: U87-MG , SK-N-SH , IMR32 , SK-N-AS

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.69 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MRPL42

Target: MRPL42 (Mitochondrial Ribosomal Protein L42 (MRPL42))

Alternative Name: mitochondrial ribosomal protein L42

Background: Mitochondrial ribosomal protein L42 , HSPC204 , L31MT , L42MT , MRP-L31 , MRP-L42 , MRP-S32 , MRPL31 , MRPS32 , PTD007 , RPML31 , S32MT,Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Further experiments will be needed to identify the specific subunit localization. Sequence analysis identified three transcript variants that encode two different isoforms. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q. [provided by RefSeq]

Molecular Weight: 17 kDa

Gene ID: 28977

UniProt: Q9Y6G3