RPE65 antibody

Catalog No. ABIN7467624

This Rabbit Polyclonal antibody specifically detects RPE65 in WB, IF, ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for RPE65 antibody (ABIN7467624)

Target: RPE65 (Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPE65 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RPE65 Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human RPE65. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.36 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for RPE65

Target: RPE65 (Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65))

Alternative Name: retinoid isomerohydrolase RPE65

Background: Retinoid isomerohydrolase RPE65 , BCO3 , LCA2 , RP20 , mRPE65 , p63 , rd12 , sRPE65,This gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa. [provided by RefSeq]

Molecular Weight: 61 kDa

Gene ID: 6121

UniProt: Q16518