WDYHV1 antibody

Catalog No. ABIN7467823

Product Details anti-WDYHV1 Antibody

Target: WDYHV1 (WDYHV Motif Containing 1 (WDYHV1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WDYHV1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF)

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human WDYHV1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.58 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for WDYHV1

Target: WDYHV1 (WDYHV Motif Containing 1 (WDYHV1))

Alternative Name: WDYHV motif containing 1 (WDYHV1 Products)

Synonyms: ntaq1 antibody, C8orf32 antibody, 2410187C16Rik antibody, AU014961 antibody, AW550036 antibody, Ntaq1 antibody, RGD1311362 antibody, C14H8orf32 antibody, si:rp71-45k5.3 antibody, WDYHV motif containing 1 antibody, WDYHV motif containing 1 S homeolog antibody, WDYHV1 antibody, wdyhv1.S antibody, wdyhv1 antibody, Wdyhv1 antibody

Background: WDYHV motif containing 1 , C8orf32,Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.

Molecular Weight: 24 kDa

Gene ID: 55093

UniProt: Q96HA8