EEFSEC antibody

Catalog No. ABIN7467832

The Rabbit Polyclonal anti-EEFSEC antibody has been validated for IHC (p), ICC and IF. It is suitable to detect EEFSEC in samples from Human.

Quick Overview for EEFSEC antibody (ABIN7467832)

Target: EEFSEC (Eukaryotic Elongation Factor, Selenocysteine-tRNA-Specific (EEFSEC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEFSEC antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-EEFSEC Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human SELB. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.55 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for EEFSEC

Target: EEFSEC (Eukaryotic Elongation Factor, Selenocysteine-tRNA-Specific (EEFSEC))

Alternative Name: eukaryotic elongation factor, selenocysteine-tRNA specific

Background: Eukaryotic elongation factor, selenocysteine-tRNA specific , EFSEC , SELB,Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP.

Molecular Weight: 65 kDa

Gene ID: 60678

UniProt: P57772