GAPDH antibody (HRP)

Catalog No. ABIN7467884

The HRP-conjugated Mouse Monoclonal anti-GAPDH antibody (Clone GT239) (ABIN7467884) specifically detects GAPDH in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for GAPDH antibody (HRP) (ABIN7467884)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: GT239

Product Details anti-GAPDH Antibody (HRP)

Cross-Reactivity: Hamster, Human, Mouse, Rat

Purification: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.

Isotype: IgG2b

Application Details

Application Notes: WB: 1:5000-1:20000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.62 mg/mL

Buffer: 0.05M Tris ( pH 7.4), 0.15M NaCl, 1 % BSA, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: glyceraldehyde-3-phosphate dehydrogenase

Background: Glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP,The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]

Molecular Weight: 36 kDa

Gene ID: 2597

UniProt: P04406