ARID5B antibody

Catalog No. ABIN7467982

The Rabbit Polyclonal anti-ARID5B antibody is suitable to detect ARID5B in samples from Human. It has been validated for WB, ICC, IF and IHC (p).

Quick Overview for ARID5B antibody (ABIN7467982)

Target: ARID5B (AT Rich Interactive Domain 5B (MRF1-Like) (ARID5B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARID5B antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ARID5B Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ARID5B. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa nuclear extract Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.63 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ARID5B

Target: ARID5B (AT Rich Interactive Domain 5B (MRF1-Like) (ARID5B))

Alternative Name: AT-rich interaction domain 5B

Background:

Synonyms: AT-rich interaction domain 5B , DESRT , MRF-2 , MRF2

Background: This gene encodes a member of the AT-rich interaction domain (ARID) family of DNA binding proteins. The encoded protein forms a histone H3K9Me2 demethylase complex with PHD finger protein 2 and regulates the transcription of target genes involved in adipogenesis and liver development. This gene also plays a role in cell growth and differentiation of B-lymphocyte progenitors, and single nucleotide polymorphisms in this gene are associated with acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]

Molecular Weight: 132 kDa

Gene ID: 84159

UniProt: Q14865

Pathways: Platelet-derived growth Factor Receptor Signaling