ATG10 antibody

Catalog No. ABIN7468028

The Rabbit Polyclonal anti-ATG10 antibody (ABIN7468028) specifically detects ATG10 in WB and IP. The antibody is reactive with Human samples.

Quick Overview for ATG10 antibody (ABIN7468028)

Target: ATG10 (Autophagy Related 10 (ATG10))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG10 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-ATG10 Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Full length human ATG10 Recombinant protein.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: human Atg10-transfected 293T cells

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ATG10

Target: ATG10 (Autophagy Related 10 (ATG10))

Alternative Name: autophagy related 10

Background:

Synonyms: autophagy related 10 , APG10 , APG10L , pp12616

Background: Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A, MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM]

Molecular Weight: 25 kDa

Gene ID: 83734

UniProt: Q9H0Y0

Pathways: ER-Nucleus Signaling, Autophagy