ATG12 antibody

Catalog No. ABIN7468031

The Mouse Monoclonal anti-ATG12 antibody (Clone GT166) (ABIN7468031) specifically detects ATG12 in WB, IF, ICC and PLA. The antibody is reactive with Human samples.

Quick Overview for ATG12 antibody (ABIN7468031)

Target: ATG12 (Autophagy Related 12 (ATG12))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATG12 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Proximity Ligation Assay (PLA)

Grade: KO Validated

Clone: GT166

Product Details anti-ATG12 Antibody

Cross-Reactivity: Human, Mouse

Purification: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ATG12. The exact sequence is proprietary.

Isotype: IgG2b

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ATG12

Target: ATG12 (Autophagy Related 12 (ATG12))

Alternative Name: autophagy related 12

Background:

Synonyms: autophagy related 12 , APG12 , APG12L , FBR93 , HAPG12

Background: Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM]

Molecular Weight: 15 kDa

Gene ID: 9140

UniProt: O94817

Pathways: Autophagy