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ATG12 antibody

This Mouse Monoclonal antibody specifically detects ATG12 in WB, IF and ICC. It exhibits reactivity toward Human.
Catalog No. ABIN7468032

Quick Overview for ATG12 antibody (ABIN7468032)

Target

See all ATG12 Antibodies
ATG12 (Autophagy Related 12 (ATG12))

Reactivity

  • 73
  • 36
  • 26
  • 6
  • 6
  • 6
  • 6
  • 5
  • 4
  • 4
  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
Human

Host

  • 71
  • 18
  • 2
Mouse

Clonality

  • 68
  • 23
Monoclonal

Conjugate

  • 50
  • 6
  • 5
  • 5
  • 4
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This ATG12 antibody is un-conjugated

Application

  • 68
  • 42
  • 30
  • 24
  • 15
  • 13
  • 13
  • 10
  • 9
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade

KO Validated

Clone

GT948
  • Cross-Reactivity

    Human, Mouse

    Purification

    Affinity purified by Protein G.

    Immunogen

    Recombinant protein corresponding to human ATG12

    Isotype

    IgG2a
  • Application Notes

    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    Comment

    Positive Control: 293T , A431 , HeLa , HepG2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 Validation: KO/KD, Orthogonal

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    PBS, No Preservative

    Preservative

    Without preservative

    Storage

    4 °C,-20 °C

    Storage Comment

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Target

    ATG12 (Autophagy Related 12 (ATG12))

    Alternative Name

    autophagy related 12

    Background

    Synonyms: autophagy related 12 , APG12 , APG12L , FBR93 , HAPG12

    Background: Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM]

    Molecular Weight

    15 kDa

    Gene ID

    9140

    UniProt

    O94817

    Pathways

    Autophagy
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