CD59 antibody

Catalog No. ABIN7468116

This Rabbit Polyclonal antibody specifically detects CD59 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CD59 antibody (ABIN7468116)

Target: CD59

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD59 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD59 Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human CD59. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: K562 , HL-60

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for CD59

Target: CD59

Alternative Name: CD59 molecule (CD59 blood group)

Background:

Synonyms: CD59 Molecule (CD59 blood group) , 16.3A5 , 1F5 , EJ16 , EJ30 , EL32 , G344 , HRF-20 , HRF20 , MAC-IP , MACIF , MEM43 , MIC11 , MIN1 , MIN2 , MIN3 , MIRL , MSK21 , p18-20

Background: This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq]

Molecular Weight: 14 kDa

Gene ID: 966

UniProt: P13987

Pathways: Complement System