DIAPH3 antibody

Catalog No. ABIN7468256

This anti-DIAPH3 antibody is a Rabbit Polyclonal antibody detecting DIAPH3 in WB and IHC (p). Suitable for Human.

Quick Overview for DIAPH3 antibody (ABIN7468256)

Target: DIAPH3 (Diaphanous Homolog 3 (Drosophila) (DIAPH3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIAPH3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DIAPH3 Antibody

Cross-Reactivity: Human, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human DIAPH3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.13 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for DIAPH3

Target: DIAPH3 (Diaphanous Homolog 3 (Drosophila) (DIAPH3))

Alternative Name: diaphanous related formin 3

Background:

Synonyms: diaphanous related formin 3 , AN , AUNA1 , DIA2 , DRF3 , NSDAN , diap3 , mDia2

Background: Binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics.

Molecular Weight: 137 kDa

Gene ID: 81624

UniProt: Q9NSV4