RAD51 antibody (AA 1-338)

Catalog No. ABIN7468272

This Mouse Monoclonal antibody specifically detects RAD51 in WB, IHC, IP, IHC (p), IF, ICC and PLA. It exhibits reactivity toward Human.

Quick Overview for RAD51 antibody (AA 1-338) (ABIN7468272)

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAD51 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC), Proximity Ligation Assay (PLA)

Grade: KO Validated

Clone: 14B4

Product Details anti-RAD51 Antibody

Binding Specificity: AA 1-338

Cross-Reactivity: Chicken, Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Full length (amino acids 1-338) Rad51 expressed in E. coli.

Isotype: IgG2b

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2 Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for RAD51

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Alternative Name: RAD51 recombinase

Background:

Synonyms: RAD51 recombinase , BRCC5 , FANCR , HRAD51 , HsRad51 , HsT16930 , MRMV2 , RAD51A , RECA

Background: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

Molecular Weight: 37 kDa

Gene ID: 5888

UniProt: Q06609

Pathways: DNA Damage Repair