Double-Strand Break Repair Protein MRE11 (MRE11) (AA 182-582) antibody

Catalog No. ABIN7468277

The Mouse Monoclonal anti-Double-Strand Break Repair Protein MRE11 antibody (Clone 12D7) (ABIN7468277) specifically detects Double-Strand Break Repair Protein MRE11 in ICC, IF, IP, WB, IHC (p), ELISA, Func and PLA. The antibody is reactive with Human samples.

Quick Overview for Double-Strand Break Repair Protein MRE11 (MRE11) (AA 182-582) antibody (ABIN7468277)

Target: Double-Strand Break Repair Protein MRE11 (MRE11)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Functional Studies (Func), Proximity Ligation Assay (PLA)

Grade: KO Validated

Clone: 12D7

Product Details

Binding Specificity: AA 182-582

Cross-Reactivity: Human, Rat

Purification: Protein G purified

Immunogen: Amino acids 182-582 of Mre11 expressed in E. coli.

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa , Jurkat , Raji , NCI-H929 , 293T , human Mre11-transfected 293T , PC-12 Validation: Comparison, KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.45 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details

Target: Double-Strand Break Repair Protein MRE11 (MRE11)

Alternative Name: MRE11 homolog, double strand break repair nuclease

Background:

Synonyms: MRE11 homolog, double strand break repair nuclease , ATLD , HNGS1 , MRE11A , MRE11B

Background: This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog, this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Molecular Weight: 81 kDa

Gene ID: 4361

UniProt: P49959