IFT57 antibody

Catalog No. ABIN7468495

Product Details anti-IFT57 Antibody

Target: IFT57 (Intraflagellar Transport 57 Homolog (IFT57))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFT57 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human HIPPI. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: H1299

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.52 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for IFT57

Target: IFT57 (Intraflagellar Transport 57 Homolog (IFT57))

Alternative Name: intraflagellar transport 57 (IFT57 Products)

Background:

Synonyms: intraflagellar transport 57 , ESRRBL1 , HIPPI , MHS4R2 , OFD18

Background: Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator, however the relevance of such function remains unclear.

Molecular Weight: 49 kDa

Gene ID: 55081

UniProt: Q9NWB7

Pathways: Hedgehog Signaling, Positive Regulation of Endopeptidase Activity