NDC80 antibody (pSer55)
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- Target See all NDC80 Antibodies
- NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))
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Binding Specificity
- pSer55
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NDC80 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Cross-Reactivity
- Human, Kangaroo rat (Dipodomys sp.), Mouse, Rat
- Purification
- Purified by antigen-affinity chromatography.
- Grade
- KO Validated
- Immunogen
- Carrier-protein conjugated synthetic peptide surrounding phospho Ser55 of human Hec1. The exact sequence is proprietary.
- Isotype
- IgG
- Top Product
- Discover our top product NDC80 Primary Antibody
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- Application Notes
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Recommended Starting Dilutions:
For WB: 1:500-1:3000
For IP: Use at a dilution of 2-4 μg/mL for 1 mg cell extract
For ICC/IF: Use at a dilution of 1:300
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher. - Comment
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Positive Control: HeLa , Blocking peptide (GTX70017-PEP) , HeLa (100 ng/ml Nocodazole treatment for 24 hr) Validation: KO/KD, Orthogonal
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.26 mg/mL
- Buffer
- 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Target
- NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))
- Alternative Name
- NDC80 kinetochore complex component (NDC80 Products)
- Synonyms
- CG9938 antibody, Dmel\\CG9938 antibody, Hec1 antibody, NDC80 antibody, dNdc80 antibody, dmNdc80 antibody, hec1 antibody, KNTC2 antibody, ndc10 antibody, tid3 antibody, 2610020P18Rik antibody, AV139730 antibody, HEC antibody, HEC1 antibody, Kntc2 antibody, kntc2l antibody, wu:fb52e02 antibody, zgc:111801 antibody, HsHec1 antibody, TID3 antibody, hsNDC80 antibody, kntc2 antibody, NDC80, kinetochore complex component antibody, CG9938 gene product from transcript CG9938-RA antibody, spindle pole body protein Ndc80 antibody, kinetochore protein Ndc80 antibody, NDC80 homolog, kinetochore complex component (S. cerevisiae) antibody, NDC80 kinetochore complex component antibody, NDC80 kinetochore complex component S homeolog antibody, NDC80 antibody, Ndc80 antibody, ndc80 antibody, ndc80.S antibody
- Background
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Synonyms: NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80
Background: HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]
- Molecular Weight
- 80 kDa
- Gene ID
- 10403
- UniProt
- O14777
- Pathways
- Maintenance of Protein Location
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