Negative Elongation Factor E (NELFE) antibody

Catalog No. ABIN7468649

The Mouse Monoclonal anti-Negative Elongation Factor E antibody (Clone E1C1) (ABIN7468649) specifically detects Negative Elongation Factor E in WB. The antibody is reactive with Human samples.

Quick Overview for Negative Elongation Factor E (NELFE) antibody (ABIN7468649)

Target: Negative Elongation Factor E (NELFE)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Western Blotting (WB)

Clone: E1C1

Product Details

Cross-Reactivity: Human

Purification: Affinity purified by Protein G.

Immunogen: The immunogen used to generate this antibody corresponds to human NELF-E.

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NELF-E-transfected 293T cells

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.52 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details

Target: Negative Elongation Factor E (NELFE)

Alternative Name: negative elongation factor complex member E

Background:

Synonyms: negative elongation factor complex member E , D6S45 , NELF-E , RD , RDBP , RDP

Background: The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins, however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq, Jul 2008]

Molecular Weight: 43 kDa

Gene ID: 7936

UniProt: P18615