RAD50 antibody (AA 1-425)

Catalog No. ABIN7468849

The Mouse Monoclonal anti-RAD50 antibody has been validated for WB, IHC, IP, ICC, IF, IHC (p) and in vitro. It is suitable to detect RAD50 in samples from Human.

Quick Overview for RAD50 antibody (AA 1-425) (ABIN7468849)

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAD50 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), In vitro Assay (in vitro)

Grade: KO Validated

Clone: 13B3

Product Details anti-RAD50 Antibody

Binding Specificity: AA 1-425

Cross-Reactivity: Human, Monkey, Mouse, Rat

Purification: Affinity purified by Protein G.

Immunogen: Amino acids 1-425 of Rad50 expressed in E. coli.

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa , HeLa nuclear extract Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.24 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for RAD50

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Alternative Name: RAD50 double strand break repair protein

Background:

Synonyms: RAD50 double strand break repair protein , NBSLD , RAD502 , hRad50

Background: The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010]

Molecular Weight: 154 kDa

Gene ID: 10111

UniProt: Q92878

Pathways: DNA Damage Repair, Protein targeting to Nucleus