ROBO1 antibody (N-Term)

Catalog No. ABIN7468914

This Rabbit Polyclonal antibody specifically detects ROBO1 in WB, IF, IHC (p) and ICC. It exhibits reactivity toward Human.

Quick Overview for ROBO1 antibody (N-Term) (ABIN7468914)

Target: ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ROBO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-ROBO1 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Chicken, Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human ROBO1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: MDA-Mb-361 , HCC1937 LROBO1 (Lentivirus infection carry human ROBO1) , HeLa membrane fraction Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ROBO1

Target: ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))

Alternative Name: roundabout guidance receptor 1

Background:

Synonyms: roundabout guidance receptor 1 , DUTT1 , SAX3

Background: Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Molecular Weight: 181 kDa

Gene ID: 6091

UniProt: Q9Y6N7

Pathways: Positive Regulation of Endopeptidase Activity