SEC61B antibody (N-Term)

Catalog No. ABIN7468931

This anti-SEC61B antibody is a Rabbit Polyclonal antibody detecting SEC61B in WB and IP. Suitable for Human.

Quick Overview for SEC61B antibody (N-Term) (ABIN7468931)

Target: SEC61B (Sec61 beta Subunit (SEC61B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SEC61B antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-SEC61B Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human SEC61B. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: mouse liver , 293T , A431 , HeLa , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.77 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for SEC61B

Target: SEC61B (Sec61 beta Subunit (SEC61B))

Alternative Name: Sec61 translocon beta subunit

Background:

Synonyms: Sec61 translocon beta subunit

Background: The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript. [provided by RefSeq]

Molecular Weight: 10 kDa

Gene ID: 10952

UniProt: P60468

Pathways: Protein targeting to Nucleus