TRIB3 antibody

Catalog No. ABIN7469086

The Rabbit Polyclonal anti-TRIB3 antibody is suitable to detect TRIB3 in samples from Human. It has been validated for WB, IHC (p), IF and ICC.

Quick Overview for TRIB3 antibody (7469086)

Target: TRIB3 (Tribbles Homolog 3 (Drosophila) (TRIB3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-TRIB3 Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human TRIB3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HUVEC Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for TRIB3

Target: TRIB3 (Tribbles Homolog 3 (Drosophila) (TRIB3))

Alternative Name: tribbles pseudokinase 3

Background:

Synonyms: tribbles pseudokinase 3 , C20orf97 , NIPK , SINK , SKIP3 , TRB3

Background: The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. [provided by RefSeq]

Molecular Weight: 40 kDa

Gene ID: 57761

UniProt: Q96RU7

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha