ASXL1 antibody (N-Term)

Catalog No. ABIN7469244

This Rabbit Polyclonal antibody specifically detects ASXL1 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for ASXL1 antibody (N-Term) (ABIN7469244)

Target: ASXL1 (Additional Sex Combs Like 1 (ASXL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASXL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Product Details anti-ASXL1 Antibody

Binding Specificity: N-Term

Specificity: Knockdown/Knockout validation was supported by references (PMID:26385183)

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human ASXL1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: GFP-human ASXL1-transfected 293T cells Validation: KO/KD, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ASXL1

Target: ASXL1 (Additional Sex Combs Like 1 (ASXL1))

Alternative Name: ASXL1

Background:

Synonyms: ASXL transcriptional regulator 1 , BOPS , MDS

Background: This gene is similar to the Drosophila additional sex combs gene, which encodes a chromatin-binding protein required for normal determination of segment identity in the developing embryo. The protein is a member of the Polycomb group of proteins, which are necessary for the maintenance of stable repression of homeotic and other loci. The protein is thought to disrupt chromatin in localized areas, enhancing transcription of certain genes while repressing the transcription of other genes. The protein encoded by this gene functions as a ligand-dependent co-activator for retinoic acid receptor in cooperation with nuclear receptor coactivator 1. Mutations in this gene are associated with myelodysplastic syndromes and chronic myelomonocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Molecular Weight: 165 kDa

Gene ID: 171023

UniProt: Q8IXJ9

Pathways: Retinoic Acid Receptor Signaling Pathway