RHEB antibody

Catalog No. ABIN7469570

The Rabbit Polyclonal anti-RHEB antibody (ABIN7469570) specifically detects RHEB in WB. The antibody is reactive with Human samples.

Quick Overview for RHEB antibody (ABIN7469570)

Target: RHEB (Ras Homolog Enriched in Brain (RHEB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHEB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RHEB Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Rheb. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for RHEB

Target: RHEB (Ras Homolog Enriched in Brain (RHEB))

Alternative Name: Rheb

Background:

Synonyms: Ras homolog, mTORC1 binding , RHEB2

Background: This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq]

Molecular Weight: 20 kDa

Gene ID: 6009

UniProt: Q15382

Pathways: RTK Signaling