RbAp48 + RbAp46 (AA 1-425) antibody

Catalog No. ABIN7469571

This Mouse Monoclonal antibody specifically detects RbAp48 + RbAp46 in EMSA, ICC, IF, IHC (p), IP and WB. It exhibits reactivity toward Human.

Quick Overview for RbAp48 + RbAp46 (AA 1-425) antibody (ABIN7469571)

Target: RbAp48 + RbAp46

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Electrophoretic Mobility-Shift Assay (EMSA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)

Clone: 15G12

Product Details

Binding Specificity: AA 1-425

Cross-Reactivity: Human, Mouse

Purification: Protein G purified

Immunogen: The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.

Isotype: IgG2a

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 0.2 - 1 μg/mL. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: K562 , THP-1 , HL-60

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details

Target: RbAp48 + RbAp46

Background: RbAp48-15G12 recognizes full length RbAp48 (48 kDa) and RbAp46 (46 kDa), related nuclear proteins first isolated by their ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). These two proteins are highly homologous at the amino acid level and are related functionally to members of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1. Both RbAp48 and RbAp46 interact with HDAC1 and mSin3 in vivo.

Molecular Weight: 46 and 48 kDa