Recombinant Liver Arginase antibody (AA 300-400)

Catalog No. ABIN7477377

This Rabbit Monoclonal antibody specifically detects Liver Arginase in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant Liver Arginase antibody (AA 300-400) (ABIN7477377)

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Liver Arginase antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-511R

Product Details anti-Liver Arginase Antibody

Binding Specificity: AA 300-400

Immunogen: Recombinant human ARG1 protein fragment (aa300-400) (Exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Liver: A strong staining should be seen in hepatocytes. Spleen: A moderate t strong positivity should be seen in granulocytes.

Negative Control: Colon: Epithelial cells, smooth muscle and the vast majority of stroma cells should stain negative (few granulocytes may stain positive).

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Liver Arginase

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Alternative Name: Arginase-1

Background: Arginase 1, ARG1, liver-type arginase, type I arginase,Arginase-1 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P05089

Pathways: Cellular Response to Molecule of Bacterial Origin