Recombinant CD5 antibody (AA 32-372)
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- Target See all CD5 Antibodies
- CD5
- Antibody Type
- Recombinant Antibody
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Binding Specificity
- AA 32-372
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This CD5 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC)
- Immunogen
- Recombinant fragment (around aa32-372) corresponding to human CD5 protein (extracellular).
- Clone
- MSVA-005R
- Isotype
- IgG
- Top Product
- Discover our top product CD5 Primary Antibody
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anti-CD5 (CD5) (AA 105-210) antibody
CD5 Reactivity: Human WB Host: Goat Polyclonal unconjugated
anti-CD5 (CD5) (AA 269-366) antibodyCD5 Reactivity: Human FACS, WB, IHC, IF, StM Host: Mouse Monoclonal CD5-2416 unconjugated
anti-CD5 (CD5) (AA 25-372) antibodyCD5 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-CD5 (CD5) antibodyCD5 Reactivity: Human FACS, WB, IHC, IP, ICC Host: Mouse Monoclonal L17F12 unconjugated
anti-CD5 (CD5) (AA 27-233) antibodyCD5 Reactivity: Human FACS, WB, ELISA Host: Mouse Monoclonal 6A11 unconjugated
anti-CD5 (CD5) (AA 403-495) antibodyCD5 Reactivity: Human, Mouse FACS, WB, IHC, ELISA Host: Mouse Monoclonal 6C3B2 unconjugated
anti-CD5 (CD5) (AA 269-366) antibodyCD5 Reactivity: Human FACS, IHC, IF, StM Host: Mouse Monoclonal CD5-2418 unconjugated
anti-CD5 (CD5) antibodyCD5 Reactivity: Human FACS, WB, IP, ELISA, IHC (fro) Host: Mouse Monoclonal CRIS1 unconjugated
anti-CD5 (CD5) antibodyCD5 Reactivity: Human FACS, WB, IP, ELISA, IHC (p) Host: Mouse Monoclonal MEM-32 unconjugated
anti-CD5 (CD5) antibody (PE)CD5 Reactivity: Human FACS Host: Mouse Monoclonal L17F12 PE
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- Application Notes
- IHC 1:100-1:200
- Comment
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Positive Control: Tonsil: A strong CD5 staining of virtually all T-cells in both T-zones and in germinal centres as well as an at least weak to moderate staining of dispersed B-cells in the mantle zone should be seen.
Negative Control: Tonsil: CD5 staining should be absent in germinal centre B-cells and in surface epithelial cells (A fraction of crypt epithelial cells may stain positive).
- Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Target
- CD5
- Alternative Name
- CD5 (CD5 Products)
- Synonyms
- CD5 antibody, Ly-1 antibody, Ly-12 antibody, Ly-A antibody, Lyt-1 antibody, LEU1 antibody, T1 antibody, CD5 molecule antibody, CD5 antigen antibody, CD5 molecule like antibody, Cd5 molecule antibody, CD5 antibody, Cd5 antibody, CD5L antibody
- Background
- CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5,CD5 antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- P06127
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