Recombinant CD7 antibody

Catalog No. ABIN7477397

The Rabbit Monoclonal anti-CD7 antibody has been validated for IHC. It is suitable to detect CD7 in samples from Human.

Quick Overview for Recombinant CD7 antibody (ABIN7477397)

Target: CD7

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD7 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-007R

Product Details anti-CD7 Antibody

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Tonsil: A moderate to strong, predominantly membranous staining of T-cells in the interfollicular zones and the germinal centres should be seen. Colon: A moderate to strong staining of the intra-epithelial T-cells in the colon mucosa should be seen.

Negative Control: Tonsil/Colon: All epithelial structures should stain negative.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for CD7

Target: CD7

Alternative Name: CD7

Background: GP40, Leu9, p41, T-cell leukemia antigen, T-cell surface antigen Leu-9, Tp40, TP41,CD7 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P09564

Pathways: Cell-Cell Junction Organization