Recombinant CD8 antibody (AA 135-235)

Catalog No. ABIN7477399

This anti-CD8 antibody is a Rabbit Monoclonal antibody detecting CD8 in IHC. Suitable for Human.

Quick Overview for Recombinant CD8 antibody (AA 135-235) (ABIN7477399)

Target: CD8

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD8 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-008R

Product Details anti-CD8 Antibody

Binding Specificity: AA 135-235

Immunogen: Synthetic peptide corresponding to CD8α residues within aa135-235 of CD8α

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Tonsil (interfollicular areas)

Negative Control: Epithelial tissues, majority of cells in germinal centres of lymphatic organs

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for CD8

Target: CD8

Alternative Name: CD8

Background: CD8 antigen, alpha polypeptide (p32), T8/Leu-2 T-lymphocyte differentiation antigen, Ly3, LYT3, MAL, T-cell surface glycoprotein CD8 alpha chain,CD8 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P01732