Recombinant CD8 antibody (AA 135-235)
Quick Overview for Recombinant CD8 antibody (AA 135-235) (ABIN7470580)
Target
See all CD8 AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
Clone
-
-
Binding Specificity
- AA 135-235
-
Purpose
- CD8 antibody (008R)
-
Immunogen
- Synthetic peptide corresponding to CD8α residues within aa135-235 of CD8α
-
Isotype
- IgG
-
-
-
-
Application Notes
- IHC 1:100-1:200
-
Comment
-
Positive Control: Tonsil (interfollicular areas)
Product Description: CD8 antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Epithelial tissues, majority of cells in germinal centres of lymphatic organs
-
Protocol
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Storage
- 4 °C,-20 °C,-80 °C
-
Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.
-
Expiry Date
- 24 months
-
-
- CD8
-
Alternative Name
- CD8
-
Background
- Gene Alias: CD8 antigen, alpha polypeptide (p32), T8/Leu-2 T-lymphocyte differentiation antigen, Ly3, LYT3, MAL, T-cell surface glycoprotein CD8 alpha chain
-
UniProt
- P01732
Target
-