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Recombinant CD34 antibody (full length)

This anti-CD34 antibody is a Rabbit Monoclonal antibody detecting CD34 in IHC. Suitable for Human.
Catalog No. ABIN7470586

Quick Overview for Recombinant CD34 antibody (full length) (ABIN7470586)

Target

See all CD34 Antibodies
CD34

Antibody Type

Recombinant Antibody

Reactivity

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Human

Host

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Rabbit

Clonality

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Monoclonal

Conjugate

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This CD34 antibody is un-conjugated

Application

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Immunohistochemistry (IHC)

Clone

034R
  • Binding Specificity

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    full length

    Purpose

    CD34 antibody (034R)

    Immunogen

    Recombinant full-length human HPCA1 protein

    Isotype

    IgG
  • Application Notes

    IHC 1:100-1:200

    Comment

    Positive Control: Liver: A moderate to strong, predominantly membranous staining of endothelial cells should be seen in portal vessels and periportal sinusoidal endothelial cells. Endothelial cells located more remotely from the portal field show a rapidly decreasing CD34 expression level.

    Product Description: CD34 antibody validated for immunohistochemistry on 76 different Normal Tissues

    Negative Control: Liver: Hepatocytes should always stain CD34 negative. Sinusoidal cells remote from the portal fields must also be CD34 negative.

    Protocol

    Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Storage

    4 °C,-20 °C,-80 °C

    Storage Comment

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.

    Expiry Date

    24 months
  • Target

    CD34

    Alternative Name

    CD34

    Background

    Gene Alias: Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin

    UniProt

    P28906
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