Recombinant Elastase 3B antibody (AA 82-238)

Catalog No. ABIN7470591

The Mouse Monoclonal anti-Elastase 3B antibody is suitable to detect Elastase 3B in samples from Human. It has been validated for IHC.

Quick Overview for Recombinant Elastase 3B antibody (AA 82-238) (ABIN7470591)

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Elastase 3B antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: 410M

Product Details anti-Elastase 3B Antibody

Binding Specificity: AA 82-238

Purpose: CELA3B antibody (410M)

Immunogen: Recombinant human CELA3B protein fragment (around aa 82-238) (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Pancreas: Acinar cells should show a strong CELA3B immunostaining with apical predominance.

Product Description: CELA3B antibody validated for immunohistochemistry on 76 different Normal Tissues

Negative Control: Kidney: CELA3B staining should be absent in all epithelial cells.

Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.

Expiry Date: 24 months

Target Details for Elastase 3B

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Alternative Name: CELA3B

Background: Gene Alias: Chymotrypsin like elastase family member 3B (CELA3B), ELA3B, Elastase IIIB, Protease E

UniProt: P08861