Recombinant Cytokeratin 5 antibody (AA 316-590)

Catalog No. ABIN7477433

The Mouse Monoclonal anti-Cytokeratin 5 antibody has been validated for IHC. It is suitable to detect Cytokeratin 5 in samples from Human.

Quick Overview for Recombinant Cytokeratin 5 antibody (AA 316-590) (ABIN7477433)

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 5 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-605M

Product Details anti-Cytokeratin 5 Antibody

Binding Specificity: AA 316-590

Immunogen: Recombinant fragment (around aa 316-590) of human Cytokeratin 5 (KRT5) protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Tonsil: A moderate to strong cytoplasmic KRT5 staining should be seen in virtually all squamous epithelial cells. Prostate: A strong cytoplasmic staining should be present in the majority of basal cells.

Negative Control: Tonsil: Lymphatic cells must not show any KRT5 positivity.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Cytokeratin 5

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Alternative Name: Cytokeratin 5

Background: 58 kDa Cytokeratin, CK5, Cytokeratin-5, DDD1, Epidermolysis Bullosa Simplex 2 (EBS2), Keratin 5, Keratin, Type II Cytoskeletal 5, Keratin-5, KRT5, Type-II Cytoskeletal 5, Type-II keratin Kb5,Cytokeratin 5 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P13647