Cytokeratin 13 antibody

Catalog No. ABIN7477441

This anti-Cytokeratin 13 antibody is a Mouse Monoclonal antibody detecting Cytokeratin 13 in IHC. Suitable for Human.

Quick Overview for Cytokeratin 13 antibody (ABIN7477441)

Target: Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 13 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-613M

Product Details anti-Cytokeratin 13 Antibody

Immunogen: esophageal keratins of rabbit origin

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Tonsil: All squamous epithelial cells of the surface squamous epithelium (except the basal layer) and a fraction of squamous cells of the crypts should show strong KRT13 staining.

Negative Control: Tonsil: All lymphocytes and blood vessels must not show any KRT13 staining.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Cytokeratin 13

Target: Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Alternative Name: Cytokeratin 13

Background: CK13, Cytokeratin-13, Keratin Type I Cytoskeletal 13, Keratin-13, KRT13, Type I Cytoskeletal 13, WSN2,Cytokeratin 13 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P13646