Recombinant KRT14 antibody (AA 350-472)

Catalog No. ABIN7477443

This Rabbit Monoclonal antibody specifically detects KRT14 in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant KRT14 antibody (AA 350-472) (ABIN7477443)

Target: KRT14 (Keratin 14 (KRT14))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This KRT14 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-614R

Product Details anti-KRT14 Antibody

Binding Specificity: AA 350-472

Immunogen: Recombinant human KRT14 fragment (around aa350-472) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Tonsil: A strong KRT14 immunostaining should be seen in the surface and crypt epithelium.

Negative Control: Tonsil: KRT14 immunostaining should be absent in all non-epithelial cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for KRT14

Target: KRT14 (Keratin 14 (KRT14))

Alternative Name: Cytokeratin 14

Background: CK-14, Dowling Meara, ebs3, ebs4, Epidermolysis Bullosa Simplex, Keratin Type I Cytoskeletal 14, Koebner, NFJ,Cytokeratin 14 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P02533