Desmoglein 1 antibody

Catalog No. ABIN7477459

This anti-Desmoglein 1 antibody is a Mouse Monoclonal antibody detecting Desmoglein 1 in IHC. Suitable for Human.

Quick Overview for Desmoglein 1 antibody (ABIN7477459)

Target: Desmoglein 1 (DSG1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmoglein 1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-544M

Product Details anti-Desmoglein 1 Antibody

Immunogen: Bovine desmoglein (desmosomal glyco protein I) from nasal epidermis.

Isotype: IgG

Application Details

Application Notes: IHC 1:75-1:150

Comment:

Positive Control: Tonsil: A moderate to strong desmoglein 1 immunostaining should be seen in a fraction of squamous epithelial cells.

Negative Control: Desmoglein 1 immunostaining should be absent in all non-squamous epithelial cell types.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Desmoglein 1

Target: Desmoglein 1 (DSG1)

Alternative Name: Desmoglein 1

Background: Cadherin family member 4, CDHF4, Desmoglein-1, Desmosomal glycoprotein 1, DG1, DSG1, EPKHE, EPKHIA, Pemphigus foliaceus antigen, PPKS1, SPPK1,Desmoglein 1 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: Q02413

Pathways: Cell-Cell Junction Organization