Desmoglein 3 antibody (AA 379-491)

Catalog No. ABIN7477461

This Mouse Monoclonal antibody specifically detects Desmoglein 3 in IHC. It exhibits reactivity toward Human.

Quick Overview for Desmoglein 3 antibody (AA 379-491) (ABIN7477461)

Target: Desmoglein 3 (DSG3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmoglein 3 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-543M

Product Details anti-Desmoglein 3 Antibody

Binding Specificity: AA 379-491

Immunogen: Recombinant fragment (around aa 379-491) human DSG3 protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:75-1:150

Comment:

Positive Control: Tonsil: A moderate to strong desmoglein 3 immunostaining should be seen in a fraction of squamous epithelial cells.

Negative Control: Tonsil: Desmoglein 3 immunostaining should be absent in all non-squamous epithelial cell types.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Desmoglein 3

Target: Desmoglein 3 (DSG3)

Alternative Name: Desmoglein 3

Background: 130 kDa pemphigus vulgaris antigen (PVA), Balding (Bal), Cadherin family member 6 (CDHF6), Desmoglein-3 (DSG3),Desmoglein 3 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P32926