ANO1 antibody (AA 2-101)

Catalog No. ABIN7477463

The Mouse Monoclonal anti-ANO1 antibody has been validated for IHC. It is suitable to detect ANO1 in samples from Human.

Quick Overview for ANO1 antibody (AA 2-101) (ABIN7477463)

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ANO1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-201M

Product Details anti-ANO1 Antibody

Binding Specificity: AA 2-101

Immunogen: Recombinant human DOG-1 protein fragment (aa 2-101) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Appendix: An at least moderate, predominantly membranous staining should be seen in Cajal cells in the muscular wall and a weak membranous staining should be seen in columnar epithelial cells in the basal part of the crypts of the appendix.

Negative Control: Appendix: Staining should be absent in the majority of cells in the muscular wall and in epithelial cells located at the surface.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for ANO1

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Alternative Name: Dog1

Background: Anoctamin 1, Calcium Activated Chloride Channel, Discovered On Gastrointestinal Stromal Tumors Protein 1, TAOS2, ORAOV2, TMEM16A,Dog1 antibody validated for immunohistochemistry on 76 different Normal Tissues