Recombinant EpCAM antibody (Extracellular Domain)

Catalog No. ABIN7477469

The Rabbit Monoclonal anti-EpCAM antibody has been validated for IHC. It is suitable to detect EpCAM in samples from Human.

Quick Overview for Recombinant EpCAM antibody (Extracellular Domain) (ABIN7477469)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-326R

Product Details anti-EpCAM Antibody

Binding Specificity: AA 100-224, Extracellular Domain

Immunogen: Recombinant fragment from the extracellular domain of human EpCAM protein (around aa100-224) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Kidney: In kidney, distal tubule cells must show a strong predominantly membranous staining, while at a least moderate predominantly basolateral staining must be seen in the majority of proximal tubules cells and in scattered epithelial cells lining the Bowman capsule.

Negative Control: Tonsil: EpCAM staining should be absent in lymphocytes or smooth muscle cells of the vessels.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: EpCAM

Background: Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TROP1, Tumor-associated Calcium Signal Transducer 1 (TACSTD1), ECS-1, Epidermal Surface Antigen 1, ESA1, FLOT2, Flotillin-2, Membrane Component, Chromosome 17, Surface Marker-1 (M17S1), REG-1, Reggie-1, Reggie-2,EpCAM antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P16422