FABP1 antibody (AA 1-127)
Quick Overview for FABP1 antibody (AA 1-127) (ABIN7477473)
Target
See all FABP1 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- AA 1-127
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Immunogen
- Human recombinant FABP1 protein fragment (around aa1-127) (exact sequence is proprietary)
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Isotype
- IgG
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Application Notes
- IHC 1:100-1:200
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Comment
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Positive Control: Kidney: A strong cytoplasmic FABP1 immunostaining should be seen in cells of the proximal tubule while other cell types remain negative or display only a faint staining.
Negative Control: Tonsil: All epithelial and lymphocytic cells must not show any FABP1 immunostaining.
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Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Restrictions
- For Research Use only
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Format
- Liquid
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- FABP1 (Fatty Acid Binding Protein 1, Liver (FABP1))
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Alternative Name
- FABP1
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Background
- FABP1, L-FABP,FABP1 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P07148
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Pathways
- Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha
Target
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