GP2 antibody (AA 35-179)

Catalog No. ABIN7477485

This Mouse Monoclonal antibody specifically detects GP2 in IHC. It exhibits reactivity toward Human.

Quick Overview for GP2 antibody (AA 35-179) (ABIN7477485)

Target: GP2 (Glycoprotein 2 (Zymogen Granule Membrane) (GP2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GP2 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-475M

Product Details anti-GP2 Antibody

Binding Specificity: AA 35-179

Immunogen: Recombinant fragment of human GP2 protein (around aa 35-179) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Pancreas: Acinar cells should show a strong immunostaining with apical predominance. Duodenum: Glandular cells of Brunner glands should show a weak to moderate GP2 positivity.

Negative Control: Colon: GP2 staining should be completely absent in glandular and stromal cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for GP2

Target: GP2 (Glycoprotein 2 (Zymogen Granule Membrane) (GP2))

Alternative Name: GP2

Background: Glycoprotein 2 (zymogen granule membrane), GP2, Pancreatic zymogen granule membrane associated protein GP2, Pancreatic zymogen granule membrane protein GP-2, ZAP75,GP2 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P55259