Recombinant MBP antibody (AA 150-250)
Quick Overview for Recombinant MBP antibody (AA 150-250) (ABIN7470636)
Target
See all MBP AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- AA 150-250
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Purpose
- Myelin Basic Protein antibody (390R)
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Immunogen
- Recombinant fragment (around aa 150-250) of human MBP (exact sequence is proprietary)
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Isotype
- IgG
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Application Notes
- IHC 1:100-1:200
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Comment
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Positive Control: Brain: A fibrillar staining pattern should be seen.
Product Description: Myelin Basic Protein antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Colon: MBP staining should be completely absent.
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Protocol
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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Restrictions
- For Research Use only
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Format
- Liquid
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.
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Expiry Date
- 24 months
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- MBP (Myelin Basic Protein (MBP))
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Alternative Name
- Myelin Basic Protein
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Background
- Gene Alias: GDB, Golli MBP, myelin basic protein, Hemopoietic MBP, HMBPR, HUGO, MLD, Myelin A1 Protein, basic, Myelin Deficient, Myelin membrane encephalitogenic protein, SHI, Shiverer, SP
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UniProt
- P02686
Target
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