MCM3 antibody

Catalog No. ABIN7477513

This anti-MCM3 antibody is a Mouse Monoclonal antibody detecting MCM3 in IHC. Suitable for Human.

Quick Overview for MCM3 antibody (ABIN7477513)

Target: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCM3 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-503M

Product Details anti-MCM3 Antibody

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: A strong nuclear MCM3 immunostaining should be seen in virtually all crypt base cells.

Negative Control: Colon: MCM3 immunostaining should be largely absent in surface epithelial cells and in most stroma cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for MCM3

Target: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Alternative Name: MCM3

Background: Minichromosome Maintenance Complex Component 3, DNA Polymerase Alpha Holoenzyme-Associated Protein P1, DNA Replication Licensing Factor MCM3, RLF Subunit Beta, P1-MCM3, P102,MCM3 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P25205

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA