Recombinant MSH6 antibody (AA 1-100)

Catalog No. ABIN7477533

This Rabbit Monoclonal antibody specifically detects MSH6 in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant MSH6 antibody (AA 1-100) (ABIN7477533)

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MSH6 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-906R

Product Details anti-MSH6 Antibody

Binding Specificity: AA 1-100

Immunogen: Recombinant fragment (around aa 1-100) of human MSH6 protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:75-1:150

Comment:

Positive Control: Tonsil: Virtually all mantle zone B-cells must show an at least weak to moderate nuclear staining. A moderate to strong nuclear staining must be seen in the germinal centre B-cells.

Negative Control: Colon adenocarcinoma with previously documented loss of MSH6 expression: Nuclear staining should be absent in cancer cells, while a nuclear staining must be seen in stromal and inflammatory cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for MSH6

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Alternative Name: MSH6

Background: DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, mutS (E. coli) homolog 6, MutS alpha 160 kDa subunit, MutS-alpha 160 kDa subunit, p160, Sperm associated protein,MSH6 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P52701

Pathways: DNA Damage Repair, Chromatin Binding, Production of Molecular Mediator of Immune Response