ENO2/NSE antibody (AA 416-433)
Quick Overview for ENO2/NSE antibody (AA 416-433) (ABIN7477539)
Target
See all ENO2/NSE (ENO2) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- AA 416-433
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Immunogen
- A synthetic peptide of human NSE gamma (around aa416-433) (exact sequence is proprietary)
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Isotype
- IgG
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anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-285) antibody
ENO2 Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal C10 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 251-433) antibodyENO2 Reactivity: Human, Mouse WB, IHC, ELISA, FACS Host: Mouse Monoclonal 5D3 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 325-434) antibodyENO2 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 1A3 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 416-433) antibodyENO2 Reactivity: Human, Mouse, Rat WB, IHC, StM Host: Mouse Monoclonal ENO2-1375 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-434) antibodyENO2 Reactivity: Mouse WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 1-434) antibodyENO2 Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal C12 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-434) antibodyENO2 Reactivity: Rat WB, IHC, FACS Host: Rabbit Polyclonal unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-285) antibodyENO2 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 416-433) antibodyENO2 Reactivity: Human, Mouse, Rat WB, IF, FACS, IHC (p) Host: Mouse Monoclonal ENO2-1375 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 195-434) antibodyENO2 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
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Application Notes
- IHC 1:100-1:200
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Comment
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Positive Control: In the colon, axons and ganglion cells in lamina propria and muscular wall must show at least a moderate NSE staining, while epithelial and lymphatic cells remain negative.
Negative Control: In the colon, epithelial and lymphatic cells do not show NSE staining.
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Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Restrictions
- For Research Use only
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Format
- Liquid
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
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Alternative Name
- NSE gamma
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Background
- ENO2, ENOG, Enolase 2 gamma neuronal, Enolase2, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE , -phospho-D-glycerate hydrolyase,NSE gamma antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P09104
Target
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