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Occludin antibody (AA 282-415)

This anti-Occludin antibody is a Mouse Monoclonal antibody detecting Occludin in IHC. Suitable for Human.

Membranous occludin immunostaining of respiratory epithelium scaled (Occludin antibody (AA 282-415))

Clear cell ovarian carcinoma with moderate occludin staining of tumor cells (Occludin antibody (AA 282-415))

In squamous epithelium occludin positivity is regularly seen but staining is reduced or absent in basal and superficial cell layers scaled (Occludin antibody (AA 282-415))

3 images

Catalog No. ABIN7477543

Quick Overview for Occludin antibody (AA 282-415) (ABIN7477543)

Target

See all Occludin (OCLN) Antibodies

Occludin (OCLN)

Reactivity

112
62
44
34
18
12
4
3
3
2
2
2

Human

Host

Mouse

Clonality

Monoclonal

Conjugate

55
10
6
5
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1

This Occludin antibody is un-conjugated

Application

101
35
31
30
26
26
16
16
13
12
10
6
2
1
1
1

Immunohistochemistry (IHC)

Clone

MSVA-415M

Discover our top product OCLN Primary Antibody

Binding Specificity
16

16

12

11

9

6

5

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
AA 282-415

Immunogen

ecombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)

Isotype

IgG
anti-Occludin (OCLN) (AA 431-522) antibody
OCLN Reactivity: Human, Mouse, Pig WB, ELISA, FACS, IF (cc), IF (p), IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Occludin (OCLN) (AA 263-522) antibody
OCLN Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

anti-Occludin (OCLN) (C-Term) antibody
OCLN Reactivity: Human WB, IHC (p), IHC (fro) Host: Goat Polyclonal unconjugated

anti-Occludin (OCLN) (AA 423-522) antibody
OCLN Reactivity: Human WB, ELISA, IP, IF Host: Mouse Monoclonal 1G7 unconjugated

anti-Occludin (OCLN) (AA 282-415) antibody
OCLN Reactivity: Human WB, IHC (f) Host: Mouse Monoclonal OCLN-2181 unconjugated

anti-Occludin (OCLN) (AA 1-522) antibody
OCLN Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated

anti-Occludin (OCLN) (AA 1-522) antibody
OCLN Reactivity: Human WB, PLA Host: Rabbit Polyclonal unconjugated

anti-Occludin (OCLN) (AA 285-430) antibody
OCLN Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-Occludin (OCLN) (AA 350-450) antibody
OCLN Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

anti-Occludin (OCLN) (C-Term) antibody
OCLN Reactivity: Human, Mouse, Rat, Dog WB, IHC, ELISA, IF, IHC (p) Host: Mouse Monoclonal OC-3F10 unconjugated

Application Notes

IHC 1:100-1:200

Comment

Positive Control: Colon: A strong membranous occludin staining of epithelial cells should be seen with a particularly strong staining at the apical/luminal membranes.
Negative Control: Colon: All non-epithelial and non-endothelial cells must not show any occludin staining.

Protocol

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions

For Research Use only
Format

Liquid

Storage

4 °C,-20 °C,-80 °C

Storage Comment

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
Target

Occludin (OCLN)

Alternative Name

Occludin

Background

BLCPMG, Occludin, OCLN, Phosphatase 1 regulatory subunit 115, PPP1R115, PTORCH1, Tight junction protein occludin,Occludin antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt

Q16625

Pathways

Cell-Cell Junction Organization, Hepatitis C

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