Occludin antibody (AA 282-415)

Catalog No. ABIN7470652

This anti-Occludin antibody is a Mouse Monoclonal antibody detecting Occludin in IHC. Suitable for Human.

Quick Overview for Occludin antibody (AA 282-415) (ABIN7470652)

Target: Occludin (OCLN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Occludin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: 415M

Product Details anti-Occludin Antibody

Binding Specificity: AA 282-415

Purpose: Occludin antibody (415M)

Immunogen: ecombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: A strong membranous occludin staining of epithelial cells should be seen with a particularly strong staining at the apical/luminal membranes.

Product Description: Occludin antibody validated for immunohistochemistry on 76 different Normal Tissues

Negative Control: Colon: All non-epithelial and non-endothelial cells must not show any occludin staining.

Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.

Expiry Date: 24 months

Target Details for Occludin

Target: Occludin (OCLN)

Alternative Name: Occludin

Background: Gene Alias: BLCPMG, Occludin, OCLN, Phosphatase 1 regulatory subunit 115, PPP1R115, PTORCH1, Tight junction protein occludin

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C