Recombinant UCHL1 antibody

Catalog No. ABIN7477561

This anti-UCHL1 antibody is a Rabbit Monoclonal antibody detecting UCHL1 in IHC. Suitable for Human.

Quick Overview for Recombinant UCHL1 antibody (ABIN7477561)

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This UCHL1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-905R

Product Details anti-UCHL1 Antibody

Immunogen: Recombinant full-length human UCHL1 protein

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Pancreas: Islets cells and interspersed nerve fibres should show strong PGP9.5 staining.

Negative Control: Pancreas: Acinar cells and excretory ducts should not show any PGP9.5 immunostaining.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for UCHL1

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Alternative Name: PGP9.5 / UCHL1

Background: Gracile Axonal Dystrophy, Neuron Cytoplasmic Protein 9.5, Park5, Parkinson Disease 5, PGP95, Protein Gene Product 9.5, Ubiquitin Carboxyl-terminal Esterase L1, Ubiquitin Carboxyl- terminal Hydrolase Isozyme L1, Ubiquitin Thioesterase L1, Ubiquitin Thiolesterase L1,PGP9.5 / UCHL1 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P09936

Pathways: Feeding Behaviour